Prohibitin 1 / PHB1 (AA 167-261) anticorps

N° du produit ABIN5646917

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans IHC (p), ELISA et WB. Adapté pour Humain.

Aperçu rapide pour Prohibitin 1 / PHB1 (AA 167-261) anticorps (ABIN5646917)

Antigène: Prohibitin 1 / PHB1

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)

Clone: PHB-1882

Détail du produit

Épitope: AA 167-261

Attributs du produit: This antibody recognizes a protein of 30 kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Purification: Purified

Pureté: Protein G affinity chromatography

Immunogène: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the PHB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL,ELISA: 2-4 μg for coating using the BSA-free format),Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the PHB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Prohibitin 1 / PHB1

Sujet: This antibody recognizes a protein of 30  kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.